| 1. | Taking chua ' s circuit as signal source , all kinds chaotic signal sequence can be obtained in computer 对生成的混沌序列进行了相关性分析,结果表明混沌序列保持了混沌信号的特征。 |
| 2. | With protokaryotic over - expression , the products of ppiv were identified by sds - page analysis . the signal sequence inhibits the expression in e . coli 该基因与发表的木瓜蛋白酶cdna序列对应部分同源性达98 . 8 。 |
| 3. | Signal recognition particle ( srp ) - - a particle composed of proteins and 7sl rna that binds to signal sequences and targets polypeptide chains to the endoplasmic reticulum 讯息(指挥)序列- -蛋白质末端的一段疏水性氨基酸,在细菌中引导蛋白质分泌或在真核细胞中将蛋白质并入内质网。 |
| 4. | Different correlation dimensions explain the differences of the dynamical mechanism and systematic complex level . different pressure fluctuation signal sequences have different fractal characteristics . 4 计算得出的不同关联维数反映出不同脉动压强信号序列所具有的不同分形特征,说明动力形成机制、系统复杂程度的差异。 |
| 5. | The human total rna was extracted from the foetal liver tissue sample of spontaneous abortion . mrna was separated and purified from total rna . apoe3 cdna was amplified by rt - pcr with a signal or without a signal sequence 从正常流产胎几肝组织中提取总rna ,分离纯化mrna ,以mrna为模板, rt pcr特异扩增带信号肽和不带信号肽的人apoescdna片段,将apoe |
| 6. | Sequence analysis the cdna clone contains an 546bp orf ( open reading fragment ) encoding a predicted protein of 181 amino acids with molecular weight of 20 . 7kd , 130bp 5 ' non - coding region and 152bp 3 " non - coding region including polyadenylation signal sequence aattaa and poly a tail . 3 棉花arf基因的序列分析核苷酸序列分析表明, gharf的全长为828bp ,其中含有一个从131bp到676bp间的546bp构成的一个完整开放阅读框,它编码181个氨基酸、分子量为20 . 7kd的蛋白质。 |
| 7. | Many glycoproteins of lower and higher eucaryotes are attached to the plasma membrane by means of a glycosylphosphatidylinositol ( gpi ) . gpi - anchored proteins are synthesized on membrane - bound ribosomes . upon translocation of the pro - protein across the endoplasmic reticulum membrane , gpi : protein transamidase ( gp1t ) recognize and removes the carboxy terminal gpi signal sequence and attaches a gpi molecule to the newly exposed carboxy terminal amino acid Gpi化前体蛋白在依附于膜的核糖体上合成,当其易位穿过内质网( er )膜后,被gpi :蛋白质转酰胺基酶( gpit )识别, gpit在移走其羧基端gpi信号序列的同时将gpi分子连接至新生成的氨基酸位点上。 |
| 8. | Strain bl21 , and gene expression was induced by iptg . the target proteins were directed into the periplasmic space by the staphylococcal protein a signal sequence preceding the rgd - hirudin gene . using ion exchange chromatography and gel filtration chromatography , the chimera proteins were purified , and both of them showed a single band in tricine - sds - page . the results of activity analysis suggested that these two chimera proteins not only have antithrombin activities , but gain platelet aggregation inhibitory activities as well 通过离子交换层析和凝胶过滤层分别对两种嵌合体蛋白进行纯化,纯化产物在tricine - sds - page中都显示为单一条带。活性分析结果表明两种嵌合体蛋白在保留水蛭素抗凝血酶活力的同时,还呈现抗血小板聚集活性。 |
| 9. | The signal peptide sequence which was used for secretedly expressing divergent gene in mammary gland cells was ligated to the e2 gene , the e2 gene with signal sequence was obtained by pcr . the gene resisting kanamycin was cut down from pgfp - cl vector and inserted into p22 vector , a p22 vector with gene resisting kanamycin was constructed , it was tried to construct an expression vector for transforming go 猪瘟病毒ez基因的乳腺特异表达载体构建:将在乳腺细胞中特异分泌的信号肽序列连接到ez基因, pcr得到了目的片段,再将pgfpci载体上的kana基因切下与在乳腺细胞中特异表达的p22载体连接,使其带有筛选标记,然后将带有信号肽的ez插入到p22中,试图构建山羊乳腺上皮细胞的特异性表达载体。 |
| 10. | Major difference of f gene lies in no . 1 - no . 32 amino acid residues on n - terminal , which is the signal sequence of f protein and cut off short after the new peptide of f protein is combined with the membrane of vesicle . the sequence is not the composition of the f protein and the evolutronary force it bears is very small 氨基酸的差异主要集中在n端1 - 32残基,该序列为f蛋白的信号序列,在f蛋白新生多肽与囊膜泡结合后不久被切下来,因此他不是功能性f蛋白的构成成分,所承受的进化强制就很小。 |